Packaging has always plays an important role in protecting the food products from the external environment with as aim to increase the shelf life of product and satisfy consumer requirements of safe food for consumption. In recent times a lot of research work is going on advances in packaging technologies and active packaging of food is one of the technology gaining popularity and wide acceptance for maintaining the quality attributes along with increasing the shelf life of food products.
Active packaging system make use of active materials or ingredients such as ethylene emitters (KMnO4, Titanium dioxide), CO2 emitters (citric acid, sodium bicarbonate) and O2scavengers (gallic acid, glucose oxidase); biological active molecules from plants like polyphenols, flavonoids and essential oils that helps in prolongs the shelf life and maintain the wholesomeness of food products. In recent times, researchers are focusing more on essential oils extracted from different parts of plant such leaves, roots, bark, stem, bud, flowers etc. The bioactive compounds in essential oils are reported to have antimicrobial and antioxidant properties that make them suitable for incorporating in packaging material for enhancing the shelf life of food products.
Essential oils (EO):
Essential oils are secondary metabolites of plants and are volatile, aromatic in nature with a peculiar and strong aroma inherent to oil. The EO are colorless, hydrophobic and liquid at ambient temperatures and most of the EO possess Generally Recognized as Safe (GRAS) status by American Food and Drug Administration (FDA). EO have a wide spectrum of biological activity due to the presence of constituents such as alkaloids, terpenes, flavonoids, ketones, phenols (eugenol, thymol), aldehydes (citral, benzaldehyde, cinnamaldehyde), flavones (isoflavones), and alcohols (menthol, terpineol, linalool). These active compounds are responsible for the antioxidant, antifungal, antimicrobial activities of EO. The mechanism of antimicrobial activity of EO is due the ability of EO to pass through the lipid bilayers of cell membrane there by causing electrolyte leakage and interfering the transport mechanism of ATP synthesis in mitochondria resulting in cells lysis and eventually the cell death. The other use of EO is exploited for use as an alternative to chemical additives for preserving the food from bio chemical and microbial decay. The major EO incorporated in biodegradable films and edible coatings are oregano, lemongrass, tea tree, sage, clove, cinnamon, peppermint, rosemary, thyme, lavender etc. are used for wrapping and coting of various food matrixes such as fruits and vegetables, fish and meat products, dairy products. The essential oil along with their bioactive components are listed in Table 1.
Essential oil |
Family name |
Predominate Bioactive constituents |
Plant Part |
References |
Cinnamon |
Cinnamomum zeylanicum |
Cinmenaldehyde |
Bark, Leaves |
Behbahani et al., 2020 |
Clove Oil |
Syzygium aromaticum |
Eugenol |
Flower buds |
Sharma et al., 2020 |
Thyme |
Thymus vulgaris |
Thymol, p-cymene |
Aerial parts of flower |
Sharma et al., 2020 |
Lemongrass |
Cymbopogon citratus L. |
Citral (neral and geranial) |
Leaves |
Trang et al., 2020 |
Tea Tree |
Melaleuca alternifolia |
Terpinen-4-ol |
Leaves |
Martínez etal., 2018 |
Oregano |
Origanum vulgare |
Carvacrol , Thymol |
Leaves |
Oliveira et al., 2017 |
Lemon |
Citrus limon |
Limonene |
Peels |
Rahmawati et al., 2017 |
Rosemary |
Rosmarinus officinalis L. |
1,8-cineole |
Leaves |
Raskovic et al., 2014 |
Sage |
Salvia officinalis L.
|
Camphor |
Leaves |
Abu-Darwish et al, 2013 |
Applications of EO in food packaging system:
The active packaging system consisting of polymeric films, biodegradable films and edible coatings that can act as carrier for bioactive compounds of EO and help in slow release of active compounds in food matrixes, thereby improving the food safety and stability from the food borne pathogens during storage of food products (Fig.1).
The addition of EO in films not only improves the antimicrobial, antioxidant properties but also lowers down the water permeability of film thereby modify the film characteristics. The films and coatings incorporated with essential oils for preserving food products from food born pathogen is listed in Table 2:
Essential Oil |
Film Matrix |
Food Product |
Microorganism |
References |
Tea Tree |
Chitosan |
fresh-cut bell pepper |
Salmonella enterica, and Listeria monocytogens |
Sathiyaseelan et al., 2021 |
Clove |
Pectin |
bream fillets |
E. coli |
Sharma et al., 2020 |
Rosemary |
Chitosan |
Fresh Polutry |
Bacillus cereus and Salmonella enterica |
Souza et al., 2019 |
Oregano |
Whey Protein |
Sausages |
Listeria monocytogenes |
Catarino, et al., 2017 |
Thyme |
Cassava starch |
Strawberries |
Bacillus subtilis |
Frazao et al., 2017 |
Rosemary |
Whey Protein |
Lamb Meat |
E. coli and Staphylococcus aureus |
Sani et al., 2017 |
Lemon grass |
Sodium alginate |
Fresh-cut Fuji apples |
E. coli |
Salvia-Trujillo et al., 2015 |
Cinnamon |
Pectin |
Cut Peach |
E.coli and Staphylococcus aureus |
Ayala-Zavala et al., 2013 |
Limitations of using essential oil for active food packaging:
EO have an inherent strong aroma that may have a negative effect on the sensory properties of the food products. Moreover the EO are highly volatile and possess stability issues such as poor solubility and unstable to light conditions which in turn reduces the efficacy of EO during its incorporation in packaging materials. To overcome these problems nano technological interventions such as nano encapsulation, nano emulsion and nano fibres are used for improving the efficacy of EO without affect the organoleptic properties of food products.
Future prospects
Active Packaging system consisting of EO have demonstrated the potential of improving the antioxidant, antimicrobial properties of the packaging films and coatings. The incorporation of EO in packaging films have prolonged the shelf life of food products due to the presence of bioactive components that are responsible for preventing chemical and microbial decay. Beside the advantages of EO, there is need of more researches on nano technological approaches for improving the stability of EO without effecting the organoleptic properties of the food.
References:
- Abu-Darwish, M. S., Cabral, C., Ferreira, I. V., Gonçalves, M. J., Cavaleiro, C., Cruz, M. T., Salgueiro, L. 2013. Essential oil of common sage (Salvia officinalis L.) from Jordan: Assessment of safety in mammalian cells and its antifungal and anti-inflammatory potential. BioMed Research International, 538940
- Alizadeh Behbahani, B., Falah, F., Lavi Arab, F., Vasiee, M., & Tabatabaee Yazdi, F. 2020. Chemical composition and antioxidant, antimicrobial, and antiproliferative activities of Cinnamomum zeylanicum bark essential oil. Evidence-based Complementary and Alternative Medicine, 5190603
- Sani, M.; Ehsani, A.; Hashemi, M. 2017. Whey protein isolate/cellulose nanofibre/TiO2 nanoparticle/rosemary essential oil nanocomposite film: Its effect on microbial and sensory quality of lamb meat and growth of common foodborne pathogenic bacteria during refrigeration. International Journal of .Food Microbiology. 251, 8–14
- Ayala?Zavala, J. F., Silva?Espinoza, B. A., Cruz?Valenzuela, M. R., Leyva, J. M., Ortega?Ramírez, L. A., Carrazco Lugo, D. K., Miranda, M. R. A. 2013. Pectin–cinnamon leaf oil coatings add antioxidant and antibacterial properties to fresh?cut peach. Flavour and Fragrance Journal, 28(1), 39-45.
- Catarino, M. D., Alves-Silva, J. M., Fernandes, R. P., Gonçalves, M. J., Salgueiro, L. R., Henriques, M. F., and Cardoso, S. M. 2017. Development and performance of whey protein active coatings with Origanum virens essential oils in the quality and shelf life improvement of processed meat products. Food Control. 80, 273–280.
- Frazão, G. G. S., Blank, A. F., and de Aquino Santana, L. C. L. 2017. Optimisation of edible chitosan coatings formulations incorporating Myrcia ovata Cambessedes essential oil with antimicrobial potential against foodborne bacteria and natural microflora of mangaba fruits. LWT-Food Science and Technology. 79, 1–10.
- Martínez, K., Ortiz, M., Albis, A., Gilma Gutiérrez Castañeda, C., Valencia, M. E., & Grande Tovar, C. D. 2018. The effect of edible chitosan coatings incorporated with Thymus capitatus essential oil on the shelf-life of strawberry (Fragaria x ananassa) during cold storage. Biomolecules, 8(4), 155.
- Oliveira, S. P. L. F., Bertan, L. C., De Rensis, C. M. V. B., Bilck, A. P., & Vianna, P. C. B. 2017. Whey protein-based films incorporated with oregano essential oil. Polímeros, 27, 158-164.
- Rahmawati, D., Chandra, M., Santoso, S., & Puteri, M. G. 2017. Application of lemon peel essential oil with edible coating agent to prolong shelf life of tofu and strawberry. In AIP Conference Proceedings (Vol. 1803, No. 1, p. 020037). AIP Publishing LLC.
- Raskovic, A., Milanovic, I., Pavlovic. 2014. Antioxidant activity of rosemary (Rosmarinus officinalis L.) essential oil and its hepatoprotective potential. BMC Complementary and Alternative Medicine , 14, 225
- Salvia-Trujillo, L., Rojas-Graü, A., Soliva-Fortuny, R., and Martín-Belloso, O. 2015. Physicochemical characterization and antimicrobial activity of food-grade emulsions and nanoemulsions incorporating essential oils. Food Hydrocolloids. 43, 547–556.
- Sathiyaseelan, A., Saravanakumar, K., Mariadoss, A. V. A., Ramachandran, C., Hu, X., Oh, D. H., Wang, M. H. 2021. Chitosan-tea tree oil nanoemulsion and calcium chloride tailored edible coating increase the shelf life of fresh cut red bell pepper. Progress in Organic Coatings, 151, 106010.
- Sharma, S., Barkauskaite, S., Duffy, B., Jaiswal, A. K., & Jaiswal, S. 2020. Characterization and antimicrobial activity of biodegradable active packaging enriched with clove and thyme essential oil for food packaging application. Foods, 9(8), 1117.
- Souza, V.G.L.; Pires, J.R.A.; Vieira, É.T.; Coelhoso, I.M.; Duarte, M.P.; Fernando, A.L. Activity of chitosan-montmorillonite bionanocomposites incorporated with rosemary essential oil: From in vitro assays to application in fresh poultry meat. Food Hydrocolloids 2019, 89, 241–252
- Trang, D. T., Hoang, T. K. V., Nguyen, T. T. M., Van Cuong, P., Dang, N. H., Dang, H. D., Dat, N. T. (2020). Essential oils of lemongrass (Cymbopogon citratus Stapf) induces apoptosis and cell cycle arrest in A549 lung cancer cells. BioMed Research International, 5924856